Biochem/physiol Actions

Primary Targetliver and macrophage arginase

Cell permeable: yes

General description

Cell permeable. A key intermediate in the biosynthesis of nitric oxide by constitutive nitric oxide synthase (cNOS). NOHA can be efficiently oxidized to nitric oxide and citrulline by cytochrome P450 system. A potent inhibitor of liver and macrophage arginase (K_i = 150 µM).

Cell-permeable. A key intermediate product in the biosynthesis of nitric oxide from arginine by constitutive nitric oxide synthase (cNOS). Formed by the NADPH-dependent hydroxylation of L-arginine. NOHA can be efficiently oxidized to nitric oxide and citrulline by cytochrome P450, providing an alternative biosynthetic route for the production of nitric oxide. A potent inhibitor of liver and macrophage arginase (K_i = 150 µM).

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Boucher, J.L., et al. 1994. Biochem. Biophys. Res. Commun. 203, 1614.Pufahl, R.A., and Marletta, M.A. 1993. Biochem. Biophys. Res. Commun.193, 963.Boucher, J.L., et al. 1992. Biochem. Biophys. Res. Commun.187, 880.Stuehr, D.J., et al. 1991. J. Biol. Chem.266, 6259.Wallace, G.C., et al. 1991. Biochem. Biophys. Res. Commun.176, 528.Zembowicz, A., et al. 1991. Proc. Natl. Acad. Sci. USA88, 11172.

Packaging

Packaged under inert gas

5 mg in Plastic ampoule

Reconstitution

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.

Warning

Toxicity: Standard Handling (A)